Feature | Cyclic Photophosphorylation | Noncyclic Photophosphorylation |
Energy Source | Light energy | Light energy |
Electron Flow | Electrons cycle through Photosystem I (PS I) only | Electrons flow from Photosystem II (PS II) to Photosystem I (PS I) |
ATP Production | Yes, ATP is produced | Yes, ATP is produced |
NADPH Production | No, NADPH is not produced | Yes, NADPH is produced |
Oxygen Release | No, oxygen is not released | Yes, oxygen is released as a byproduct of water-splitting |
Photosystems Involved | Only Photosystem I (PS I) | Photosystem II (PS II) and Photosystem I (PS I) |
Water Splitting | Not involved | Involves splitting of water |
Electron Transport Chain (ETC) | Involves a shorter ETC associated with PS I | Involves a longer ETC associated with both PS II and PS I |
Proton Gradient Formation | Yes, creates a proton gradient for ATP synthesis | Yes, creates a proton gradient for ATP synthesis |
Purpose | Maintains ATP levels when NADPH is not needed | Provides ATP and NADPH required for the Calvin cycle |
Location in Chloroplast | Thylakoid membranes of the chloroplast | Thylakoid membranes of the chloroplast |
Impact on Photosynthesis | Provides a mechanism to balance ATP levels | Essential for the synthesis of glucose through the Calvin Cycle |
Overall Efficiency | Less efficient for overall photosynthesis as it doesn’t produce NADPH | More efficient for overall photosynthesis as it provides both ATP and NADPH |
Photophosphorylation is a process by which light energy is used in the light-dependent reactions for photosynthesis to synthesise ATP from ADP and inorganic phosphate. This activity is an essential part of the light energy conversion into chemical energy stored in the form of ATP and occurs within the membranes of thylakoids inside chloroplasts.
Photophosphorylation is a part of the photosynthetic process by which ATP and NADPH are produced, the essential energy carriers for the subsequent cycle of Calvin. These molecules carry energy and reduce power to drive carbon dioxide into glucose and other organic compounds supporting plants' energy needs for survival and growth.
There are two different pathways of photophosphorylation producing ATP in the light reactions of photosynthesis: cyclic and noncyclic photophosphorylation. Cyclic photophosphorylation involves only PS I, and it produces ATP without the generation of NADPH or oxygen. In contrast, noncyclic photophosphorylation involves both PS II and PS I and produces both ATP and NADPH with the release of oxygen as a byproduct. Both are essential steps of light-dependent reaction, although the two processes play different roles in supporting the overall photosynthetic process.
Cyclic photophosphorylation is light-dependent and the process by which ATP is produced using only Photosystem I and involves the cyclic flow of electrons. This does not yield NADPH or oxygen and seems to be mainly implicated in replenishing ATP levels when their levels are needed more than those of NADPH.
Light Absorption: Light absorption by photosystem I excites electrons.
Electron Transport: The electrons transfer through a series of transport proteins in the thylakoid membrane.
ATP Synthesis: The energy from electron transport is used by ATP synthase to drive the synthesis of ATP.
Return of Electron: This step is completed by cycling back the electrons to Photosystem I.
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Photosystem I (PS I): This is the light-harvesting complex where electrons get excited.
Electron Transport Chain (ETC): This is a series of proteins that translocate excited electrons and generate a proton gradient.
ATP Synthesis: In this process, ATP is produced using the energy from the proton gradient.
ATP is produced through the cyclic electron flow.
Significance and Functions
ATP Supply: Provides ATP for other cellular processes.
Balancing ATP/NADPH: Maintains the level of ATP when there is excess NADPH.
Noncyclic photophosphorylation involves both photosystems, PS II and PS I, and the overall result of this process is the formation of molecules of ATP and NADPH with the release of oxygen gas. It forms a part of the light-dependent reaction of photosynthesis and provides energy and reducing power to the Calvin cycle.
Light Absorption: Light is absorbed by photosystem II and results in the splitting of the water molecule.
Electron Transport: The electrons are passed through the electron transport chain, thus creating a proton gradient.
ATP and NADPH Synthesis: The energy from this gradient of protons drives the process of synthesising ATP. These electrons reduce NADP+ into NADPH.
Oxygen Release: Water is split to release oxygen as a byproduct.
Photosystem II, PS II - A complex responsible for water-splitting and light absorption.
Photosystem I, PS I - A complex involved in producing NADPH.
Photolysis (Water Splitting): break-down of water to supply electrons
Electron Transport Chain: transport of electrons from PS II to PS I with concomitant formation of a proton gradient.
Reduction of NADP+: NADPH Formation
Noncyclic Photophosphorylation Products
ATP: Due to the electron transport chain, a proton gradient is formed.
NADPH: From the reduction of NADP+.
Oxygen: A by-product of water-splitting
ATP and NADPH Supply: The Calvin cycle is supplied with energy and reducing power.
Oxygen Production: This is necessary for respiration in aerobic organisms.
Cyclic: Mainly maintains the concentration of ATP used by a wide range of cellular processes.
Noncyclic: Produces ATP and NADPH both of which are crucial for the Calvin cycle and hence overall photosynthetic efficiency.
While cyclic photophosphorylation produces only ATP and involves Photosystem I, noncyclic photophosphorylation produces ATP, NADPH, and oxygen involving both Photosystem II and I.
Being dependent on the action of Photosystem I alone cannot reduce NADP+ to NADPH, but works only in the production of ATP.
Noncyclic photophosphorylation produces both ATP and NADPH, used in the Calvin Cycle to convert carbon dioxide into glucose.
Yes, because cyclic photophosphorylation will balance the concentration of ATP while non-cyclic photophosphorylation supplies ATP, NADPH and oxygen.
Some of the factors affecting the efficiency of photophosphorylation include light intensity, accessibility of water, and temperature. Low light or stress in the water supply can lower both the rates of cyclic and noncyclic photophosphorylation.
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