Cyclic and non-cyclic photophosphorylation are two light-driven processes that produce ATP (and sometimes NADPH) during photosynthesis. Cyclic photophosphorylation uses Photosystem I only to form ATP, while non-cyclic photophosphorylation uses both PS I and PS II to form ATP, NADPH, and O₂. Together, they maintain the ATP/NADPH balance essential for the Calvin cycle and overall photosynthetic efficiency.
This Story also Contains
Photophosphorylation is a process in which light energy is used in the light-dependent reactions to synthesise ATP from ADP and inorganic phosphate. This activity is an essential part of the light energy conversion into chemical energy stored in the form of ATP and occurs within the membranes of thylakoids inside chloroplasts.
Photophosphorylation is a part of the photosynthesis by which ATP and NADPH are produced, the essential energy carriers for the subsequent cycle of Calvin. These molecules carry energy and reduce power to drive carbon dioxide into glucose and other organic compounds supporting plants' energy needs for survival and growth.
There are two different pathways of photophosphorylation producing ATP in the light reactions of photosynthesis: cyclic and noncyclic photophosphorylation. Both are essential steps of light-dependent reaction, although the two processes play different roles in supporting the overall photosynthetic process.
Cyclic photophosphorylation is light-dependent and the process by which ATP is produced using only Photosystem I and involves the cyclic flow of electrons. This does not yield NADPH or oxygen and seems to be mainly implicated in replenishing ATP levels when their levels are needed more than those of NADPH.
Noncyclic photophosphorylation involves both photosystems, PS II and PS I, and the result is the formation of ATP and NADPH with the release of oxygen gas. It forms a part of the light-dependent reaction of photosynthesis and provides energy and reducing power to the Calvin cycle.
The steps of cyclic photophosphorylation includes:
Light Absorption: Light absorption by photosystem I excites electrons.
Electron Transport: The electrons transfer through a series of transport proteins in the thylakoid membrane.
ATP Synthesis: The energy from electron transport is used by ATP synthase to drive the synthesis of ATP.
Return of Electron: This step is completed by cycling back the electrons to Photosystem I.
The steps of non-cyclic photophosphorylation includes:
Light Absorption: Light is absorbed by photosystem II and results in the splitting of the water molecule.
Electron Transport: The electrons are passed through the electron transport chain, thus creating a proton gradient.
ATP and NADPH Synthesis: The energy from this gradient of protons drives the process of synthesising ATP. These electrons reduce NADP+ into NADPH.
Oxygen Release: Water is split to release oxygen as a byproduct.
The difference between cyclic and noncyclic photophosphorylation in given in the table below:
Feature | Cyclic Photophosphorylation | Noncyclic Photophosphorylation |
Energy Source | Light energy | Light energy |
Electron Flow | Electrons cycle through Photosystem I (PS I) only | Electrons flow from Photosystem II (PS II) to Photosystem I (PS I) |
ATP Production | ATP is produced | ATP is produced |
NADPH Production | NADPH is not produced | NADPH is produced |
Oxygen Release | Oxygen is not released | Oxygen is released as a byproduct of water-splitting |
Photosystems Involved | Only Photosystem I (PS I) | Photosystem II (PS II) and Photosystem I (PS I) |
Water Splitting | Not involved | Involves splitting of water |
Electron Transport Chain (ETC) | Involves a shorter ETC associated with PS I | Involves a longer ETC associated with both PS II and PS I |
Proton Gradient Formation | Yes, creates a proton gradient for ATP synthesis | Yes, creates a proton gradient for ATP synthesis |
Purpose | Maintains ATP levels when NADPH is not needed | Provides ATP and NADPH required for the Calvin cycle |
Location in Chloroplast | Thylakoid membranes of the chloroplast | Thylakoid membranes of the chloroplast |
Impact on Photosynthesis | Provides a mechanism to balance ATP levels | Essential for the synthesis of glucose through the Calvin Cycle |
Overall Efficiency | Less efficient for overall photosynthesis as it does not produce NADPH | More efficient for overall photosynthesis as it provides both ATP and NADPH |
Cyclic and non-cyclic photophosphorylation play different roles.
ATP Supply: Provides ATP for other cellular processes.
Balancing ATP and NADPH: Maintains the level of ATP when there is excess NADPH.
Acts in Stress: Acts as a protective mechanism under high light intensity.
ATP and NADPH: The Calvin cycle is supplied with energy and reducing power.
Oxygen Production: Releases oxygen which is necessary for respiration in aerobic organisms.
Main Pathway: Acts as a major pathway during the normal photosynthesis.
Although both pathways are different, they are interrelated in following ways:
Both pathways operate simultaneously in chloroplasts.
When the demand of ATP is more than NADPH, cyclic flow is increased to fulfill the demand.
Together they maintain the optimal ratio of ATP and NADPH i.e., 3:2 for carbon fixation.
Important topics for NEET are:
Mechanism of Cyclic and Non-cyclic Photophosphorylation
Cyclic vs Non-cyclic Photophosphorylation
Q1. Cyclic photophosphorylation occurs only when cells are in a need of
NADPH
Oxygen
ATP
Both ATP and NADPH
Correct answer: 3) ATP
Explanation:
Cyclic photophosphorylation takes place only if the plant cells need ATP.
Key Points Regarding Cyclic Photophosphorylation:
Photosystem Involvement: This process is only involved by Photosystem I.
Products: The prime product is ATP and doesn't produce NADPH or O₂ as a byproduct.
Function: It is used when the cell needs to produce more ATP for such processes as the Calvin cycle especially when NADPH is high or in low light intensity.
Cyclic photophosphorylation is important for the efficient manner in which plants can fulfil their energy needs under given circumstances.
Hence, the correct answer is option 3. ATP.
Q2. Where does Cyclic Photophosphorylation occur in chloroplasts?
Intermembrane space
Stroma lamellae
Grana lamellae
Thylakoid lumen
Correct answer: 2) Stroma lamellae
Explanation:
The chloroplasts' Stroma lamellae are where cyclic photophosphorylation takes place.
The grana, or stacked thylakoid discs, are connected via the stroma lamellae, portions of the thylakoid membranes. Photosystem I (PSI), which is found in the stroma lamellae, is the primary site of cyclic photophosphorylation.
Through the cyclic passage of electrons through PSI, ATP is produced in this process without the formation of oxygen or NADPH.
Both ATP and NADPH are produced by non-cyclic photophosphorylation, which is carried out by grana lamellae, or the stacked thylakoid membranes.
Hence, the correct answer is option 2) Stroma lamellae.
Q3. Cyclic photophosphorylation is functional under
Low light intensity
High CO2 availability
Aerobic conditions
Anaerobic conditions
Low CO2 availability
High light intensity
Choose the correct option
I, IV and V
I. III and IV
II, III and V
I and VI
Correct answer: 1) I, IV and V
Explanation:
It operates under aerobic conditions coupled with optimum light intensity in the functioning of cyclic photophosphorylation. The process mainly takes place in the thylakoid membranes of chloroplasts and involves only photosystem I, where re-excited electrons are recycled back to the photosystem, resulting in the production of ATP without oxygen or NADPH release. Multiple sources indicate that it does not operate effectively under low light intensity or anaerobic conditions.
Hence, the correct answer is option 1) I, IV, and V
Also Read:
Frequently Asked Questions (FAQs)
Being dependent on the action of Photosystem I alone cannot reduce NADP+ to NADPH, but works only in the production of ATP.
Noncyclic photophosphorylation produces both ATP and NADPH, used in the Calvin Cycle to convert carbon dioxide into glucose.
Yes, because cyclic photophosphorylation will balance the concentration of ATP while non-cyclic photophosphorylation supplies ATP, NADPH and oxygen.
Some of the factors affecting the efficiency of photophosphorylation include light intensity, accessibility of water, and temperature. Low light or stress in the water supply can lower both the rates of cyclic and noncyclic photophosphorylation.
While cyclic photophosphorylation produces only ATP and involves Photosystem I, noncyclic photophosphorylation produces ATP, NADPH, and oxygen involving both Photosystem II and I.