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    Difference Between Photosystem 1 And Photosystem 2: Explanation, Types, Steps And FAQs

    Difference Between Photosystem 1 And Photosystem 2: Explanation, Types, Steps And FAQs

    Irshad AnwarUpdated on 27 Oct 2025, 04:45 PM IST

    Photosystem I (P700) and Photosystem II (P680) are two pigment-protein complexes in chloroplasts that work together in light reactions. PSII splits water to release oxygen and form ATP, while PSI forms NADPH. Together, they power the Calvin cycle for glucose synthesis.

    This Story also Contains

    1. What are Photosystems?
    2. Structure of Photosystems
    3. Photosystem I (PSI) – Structure and Function
    4. Photosystem II (PSII) – Structure and Function
    5. Table – Difference Between Photosystem I and Photosystem II
    6. Mechanism of Photosystem Function (Z-Scheme Overview)
    7. Photosystem Variations in Different Plant Types
    8. Photosystem 1 vs Photosystem 2 NEET MCQs (With Answers & Explanations)
    Difference Between Photosystem 1 And Photosystem 2: Explanation, Types, Steps And FAQs
    Difference Between Photosystem 1 And Photosystem 2

    What are Photosystems?

    Photosystems are the pigment-protein complexes located in the thylakoid membranes of chloroplasts. They play a critical role in capturing the energy from light to drive electron transport, which results in the formation of ATP and NADPH. These are products essential for the Calvin cycle and glucose synthesis in plants. There are two types of photosystems: Photosystem 1 and Photosystem 2, which differ in their function and composition.

    Photosystems are a very basic concept both in plant physiology and bioenergetics. The significance of photosystems is not only associated with their role in the mechanism of photosynthesis but has far-reaching applications in bioengineering or biological engineering projects like artificial photosynthesis in research, photovoltaic systems development for renewable energy, etc.

    Structure of Photosystems

    The structure of photosystems is complex and highly specialised. They include the capture and conversion of light energy to the extreme. Each photosystem is the optimal structure for carrying out the light-dependent reactions as well as absorbing the light it needs during its particular range of wavelengths.

    A photosystem consists of following components:

    Component

    Functions

    Reaction centre chlorophyll

    Absorbs light energy and initiates electron transfer.

    Antenna complex

    Captures light energy from pigments and funnels it to the reaction centre.

    Primary electron complex

    Receives excited electrons from the reaction centre.

    Protein complexes

    Anchors the pigments in the membrane.

    Photosystem I (PSI) – Structure and Function

    The key features of photosystem I are:

    • Reaction center pigment: P700 (absorbs at 700 nm, far-red region).

    • Core complex: 6 to 25 chlorophyll molecules and several proteins called the core antenna complex.

    • Primary electron acceptor: An iron-sulfur protein (ferredoxin).

    • Location: Found in the stroma lamellae.

    • Reaction: NADP+ + H+ + 2e → NADPH

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    Photosystem II (PSII) – Structure and Function

    The key features of photosystem II are:

    • Reaction center pigment: P680 (absorbs at 680 nm, red region).

    • Core complex: There is significant variation in the chlorophyll and carotenoid pigments.

    • Primary Electron Acceptor: Plastoquinone.

    • Special feature: Contains Oxygen Evolving Complex (OEC) which is responsible for water-splitting and evolution of oxygen.

    • Location: Found in the thylakoid membrane, across the stack of grana.

    • Reaction: 2H2O → 4H+ + 4e + O2

    Table – Difference Between Photosystem I and Photosystem II

    The difference between photosystem I and photosystem II is included in the table below:

    Feature

    Photosystem 1 (PSI)

    Photosystem 2 (PSII)

    Reaction Center Chlorophyll

    P700

    P680

    Primary Function

    NADPH production

    Water splitting and ATP production

    Location

    Stroma lamellae

    Grana thylakoids

    Oxygen Evolution

    No

    Yes

    Electron Donor

    Plastocyanin

    Water

    Electron Acceptor

    Ferredoxin

    Plastoquinone

    Light Absorption Peaks

    700 nm

    680 nm

    Types of Light Reaction

    Later in Z-scheme

    First in Z-scheme

    Involved in Photophosphorylation

    Cyclic and Non-cyclic

    Non-cyclic only

    Mechanism of Photosystem Function (Z-Scheme Overview)

    The electron transport chain of photosystems is a cascade of redox steps that process light energy, eventually converting it into the chemical energy in the coenzymes ATP and NADPH.

    • PS II absorbs light energy of wavelength 680 nm and transfers electrons to plastoquinone (PQ).

    • Water molecules split to replace lost electrons and release oxygen.

    • Electrons donated to plastocyanin (PC) and cytochrome b6f complex.

    • PS I absorb light by P700, electrons get excited and transport to the primary electron acceptor, ferredoxin.

    • Catalysation of the formation of NADPH by the enzyme ferredoxin-NADP+ reductase.

    • ATP synthesised via ATP synthase due to proton gradient.

    Photosystem Variations in Different Plant Types

    Photosystems may have slight variations from one type of plant to the other, making some more efficient or compatible with certain environments than others.

    Plant Type

    Description of PSI and PSII

    Adaptation

    C3 Plants

    Normal structure and functioning of PSI and PSII.

    Moderate temperature efficiency

    C4 Plants

    Enhanced light capture and ATP production.

    High temperature environments

    CAM Plants

    Temporal separation of the activity of PSI and PSII

    Minimises water loss

    Photosystem 1 vs Photosystem 2 NEET MCQs (With Answers & Explanations)

    Important topics for NEET are:

    • Types of Photosystems (PSI & PSII)

    • Mechanism of Photosystem

    • PS I vs PS II

    Practice Questions for NEET

    Q1. In photosynthesis, oxygen is produced by

    1. Photosystem I from carbon dioxide.

    2. Photosystem II from carbon dioxide.

    3. Photosystem I from water.

    4. Photosystem II from water.

    Correct answer: 4) Photosystem II from water.

    Explanation:

    The splitting of water is associated with PS II. The water splits into H+, [O], and electrons. This produces oxygen, one of the net products of photosynthesis. The water splitting complex is associated with PS II, which itself is physically located on the inner side of the membrane of the thylakoid. The electrons required to replace removed electrons from photosystem I are provided by photosystem II.

    2H2O ------ 4H+ + O2 + 4e-

    Hence, the correct answer is option 4) photosystem II from water.

    Q2. PS- I comprised of

    a) p - 680, chlorophyll a - 695, p -700, chlorophyll - b and carotenoids

    b) Chlorophyll a - 670, chlorophyll a - 690, chlorophyll b and phycobilin

    1. Both a and b are correct

    2. Both a and b are incorrect

    3. a is correct

    4. b is correct

    Correct answer: 3) a is correct

    Explanation:

    Light wavelengths have the pigments affected by them divided into two; PS-I and PS-II. PS- I comprise p - 680, chlorophyll a - 695, p -700, chlorophyll - b and carotenoids PS - II consists of chlorophyll a - 670, chlorophyll a - 690, chlorophyll b and phycobilin

    Hence, the correct answer is option 3) a is correct.

    Q3. ____________is a light-harvesting complex made up of proteins and numerous chlorophyll a, chlorophyll b, and carotenoid molecules embedded in the thylakoid membrane of plants.

    1. Antenna Complex

    2. Reaction Centers

    3. Energy-fixing reaction

    4. Chemiosmosis

    Correct answer: 1) Antenna Complex

    Explanation:

    Antenna Complex is a light-harvesting complex made up of proteins and numerous chlorophyll a, chlorophyll b, and carotenoid molecules embedded in the thylakoid membrane of plants.

    The photosystem's reaction center is where energy from absorbed light is directed to excite the molecules of chlorophyll.

    Moving ions across a semipermeable membrane to produce ATP is known as chemiosmosis. Chemiosmosis is the process by which hydrogen ions move from the thylakoid space into the stroma via ATP synthase during photosynthesis.

    Hence, the correct answer is option 1) Antenna complex is a light-harvesting complex made up of proteins and numerous chlorophyll a, chlorophyll b, and carotenoid molecules embedded in the thylakoid membrane of plants.

    Also Read:

    Frequently Asked Questions (FAQs)

    Q: What are the wavelengths where Photosystem 1 and Photosystem 2 best absorb light?
    A:

    PSI best absorbs light at 700 nm, while PSII best absorbs light at 680 nm.

    Q: In which part of the chloroplast are Photosystem 1 and Photosystem 2 found?
    A:

    PSI is situated in the stroma lamellae of the thylakoid membrane. PSII, on the other hand, is located in the grana.

    Q: How do Photosystem 1 and Photosystem 2 help to generate a net yield of ATP and NADPH?
    A:

    PSII initiates the chain that produces ATP and PSI yields electrons to NADP+ to generate NADPH.

    Q: What is the main difference between Photosystem 1 and Photosystem 2?
    A:

    Photosystem 1 produces mainly NADPH, while photosystem 2 facilitates the splitting of water and the formation of ATP.

    Q: What is the significance of Photosystems in photosynthesis?
    A:

    Photosystems capture the light energy and convert it into chemical energy. This will later be in the manufacture of glucose during photosynthesis.

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