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Symbiotic And Non Symbiotic Nitrogen Fixation

Symbiotic And Non Symbiotic Nitrogen Fixation

Edited By Irshad Anwar | Updated on Aug 26, 2024 03:39 PM IST

Definition Of Nitrogen Fixation

Nitrogen fixation is the process of converting atmospheric nitrogen, N₂, to a form that can be utilised by living organisms—for example, ammonia, NH₃. Nitrogen is an element critical to life. Nitrogen forms part of the composition of amino acids, proteins, DNA, and chlorophyll.

Without available nitrogen, plants can't grow, and the entire food web—animals and humans—would be disrupted. Nitrogen fixation may occur biologically with symbiotic bacteria like leguminous plants' Rhizobium or abiotically through natural occurrences like lighting and industrial processes, for example, the Haber-Bosch process.

Types Of Nitrogen Fixation

The types of nitrogen fixation are-

Biological Nitrogen Fixation

  • In the nitrogen cycle, the process of fixation is of prime importance and needs to be understood about other processes like nitrification, assimilation, ammonification and denitrification, which provide a source of nitrogen and its recycling in the ecosystem.

Enzyme nitrogenase

  • Catalyses reduction of atmospheric nitrogen (N₂) to ammonia (NH₃)
  • Anaerobic conditions to enable nitrogen fixation to occur

Symbiotic Nitrogen Fixation

The symbiotic Nitrogen fixation is described below-

  • The plants and nitrogen-fixing bacteria are in a mutual symbiotic relationship in which these.
  • The bacteria enter the roots of the plants, and special structures developed as a response termed nodules that perform the reaction of nitrogen fixation
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Types Of Symbiotic Nitrogen Fixation

The types of symbiotic nitrogen fixation are:

Legume-Rhizobium Symbiosis

Examples: Peas, beans, clover

Non-Legume Symbiosis

Examples: Alder trees with Frankia bacteria

Process Of Symbiotic Nitrogen Fixation

  • Infection: It is a process where the infection by bacteria requires attachment and penetration of the root hair.

  • Nodule formation: Plant cells increase and form nodules.

  • Nitrogen fixation: Atmospheric nitrogen is reduced to ammonia under the influence of bacteria in the nodules.

Importance Of Symbiotic Nitrogen Fixation

  • A better understanding and manipulation of nonsymbiotic nitrogen fixation can help in sustainable agriculture and hence global food security.

  • The process avails the important plant diet from the process so that high crop plants are achieved, ensuring sustainable agriculture.

Non-Symbiotic Nitrogen Fixation

The free-living nitrogen-fixing bacteria and the cyanobacteria do not enter into any specific association with plants. They do free-living nitrogen-fixing.

They convert atmospheric nitrogen into a biologically available form because of their metabolic activities.

Types Of Non-Symbiotic Nitrogen Fixation

The different types of non-symbiotic nitrogen fixation are:

  1. Free-Living Bacteria
  2. Examples: Azotobacter, Clostridium
  3. Cyanobacteria
  4. Examples: Anabaena, Nostoc

Process Of Non-Symbiotic Nitrogen Fixation

  • Activity of enzyme: The reaction needs to be carried out in the presence of the nitrogenase enzyme.

  • Nitrogenase: It reduces atmospheric nitrogen (N₂) to ammonia (NH₃).

Importance Of Non-Symbiotic Nitrogen Fixation

The non-symbiotic nitrogen fixation is important in:

Contribution To The Nitrogen Cycle

This appears to be a significant ecological function performed within natural ecosystems because it maintains the balance of nitrogen by converting atmospheric nitrogen from its inert form to a plant-available form.

Role In Natural Ecosystems

The process helps in the maintenance of primary productivity and thus the perpetuation of the ecosystems due to the constant replenishment of nitrogen in its reduced forms.


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Frequently Asked Questions (FAQs)

1. What is nitrogen fixation and why is it important?

Nitrogen fixation is a process whereby atmospheric nitrogen—N₂—is converted to a form taken up and used by plants, mainly in the form of ammonia or other nitrogenous compounds. 

This is very important because most organisms cannot make use of atmospheric nitrogen directly; fixed nitrogen is involved in synthesizing proteins, nucleic acids, and other important molecules that support plant growth and productivity in ecosystems

2. How does symbiotic nitrogen fixation work in legumes?

It is the symbiotic mutualism of the host leguminous plant and nitrogen-fixing bacterium, Rhizobium. These bacteria infect the root hair of the leguminous and fix nitrogen. They cause the formation of nodules in the roots of some leguminous plants. 

Within these nodules, the bacteria are capable of atmospheric nitrogen fixation, which they then convert into ammonia in the presence of the nitrogenase enzyme. The plant provides carbohydrates from the plant itself and a poison-free environment to the bacteria, while they provide fixed nitrogen to the plants.

3. What are examples of non-symbiotic nitrogen-fixing bacteria?

Non-symbiotic nitrogen-fixing bacteria are as follows:

  • Azotobacter: Free-living nitrogen-fixing bacterium in neutral and alkaline soils.

  • Clostridium: An anaerobic bacterium that fixes nitrogen in the soil.

  • Cyanobacteria, Blue-Green Algae: Anabaena, Nostoc etc. fix nitrogen in aquatic environments.

4. What factors affect the efficiency of nitrogen fixation?

The efficiency of nitrogen fixation depends on several factors. It includes:

  • Oxygen Levels: High levels of oxygen in the atmosphere repress the action.

  • Soil pH: Optimum PH ranges since nitrogen-fixing bacteria grow and activity is optimal within some PH ranges.

  • Availability of Nutrients: Nitrogenase enzyme depends on the optimum availability of molybdenum iron and other nutrients.

  • Ecological Conditions: Temperature, Moisture, and light intensity may affect nitrogen-fixing organisms activity

5. How can nitrogen fixation benefit agriculture and the environment?

Nitrogen fixation serves agriculture by improving soil fertility naturally and, therefore, independently of synthetic nitrogenous fertilizer input. It will support sustainable agriculture by promoting plant growth and yield, mainly of leguminous crops, hence food security. 

Biologically, nitrogen fixation maintains the nitrogen status in an ecosystem while at the same time helping to reduce nitrogen runoff and pollution generally for healthy and resilient ecosystems.

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