CFT Full Form
What is the full form of CFT?
CFT stands for Complement Fixation Test. One of the most important conventional tests, blood serum diagnostic analysis, is used to find antibodies in a person's serum samples based on whether fixation is present or not. The main application of CFT is for illness diagnosis. The most widely used nomenclature among physicians and pharmacists around the world is the abbreviation CFT. This test has mostly been used to identify patient infections and stop the sickness from spreading. Depending on whether or not complement fixation takes place, the complement fixation test is an immunology diagnostic evaluation that can predict the presence of a certain antibody or antigen in clinical material.
Origin of CFT
Jules Bordet was the one who first came up with the concept for this test and used it to cure syphilis, a terrible illness that is incredibly common. However, August Paul von Wassermann, a German bacteriologist, Albert Neisser, a German dermatologist and venereologist, and Carl Bruck broadened the scope of CFT. Bordet-Wassermann is another name for the Wassermann response. The test is used to treat many illnesses, including liver disease.
The fundamentals of CFT testing
Only by closely examining the underlying ideas can we fully comprehend the purpose of CFT testing. The complement fixation test measures serum antibodies that do not react with antigens to produce any apparent precipitate. Therefore, using complements is essential for a good blood test. The normal serum contains large amounts of complements, which are made up of 9 necessary protein components.
Requirements for the CFT test system
Test system
Antigen: viral ag/cardiolipin/washed sheep RBCs(Red Blood Cells)
Hemolysin: Patient serum after inactivation
Complement ( Guinea Pig serum)
Signaling system RBCs from sheep-coated 4MHD hemolysin.
For incubation, use a water bath
Microscopic plate
Microtiter plate centrifuge adaptor
Barbiturates diluent ( normal saline added with calcium and magnesium)
Comparable color standards
Requirements for the indicator system of CFT
Microbial test tubes.
Plate adapters for centrifuges.
Saline solution with calcium and magnesium
Conventional colour conventions.
For incubation, use a water bath.