get app

    Differential Gene Expression Analysis - Your Complete A to Z

    BY
    Udemy

    Brochure Enquire
    Mode

    Online

    Fees

    ₹ 3299

    Quick Facts

    particular details
    Medium of instructions English
    Mode of learning Self study
    Mode of Delivery Video and Text Based

    Course and certificate fees

    Fees information
    ₹ 3,299
    certificate availability

    Yes

    certificate providing authority

    Udemy

    The syllabus

    Introduction to the Course

    • Welcome to the Course!
    • Course structure, aims and objectives
    • Your first bioinformatic challenge!
    • Quiz 1 file - an explanation
    • What is this sequence?
    • Quiz 1 walkthrough - undertaking a BLAST search

    Biology for differential gene expression analysis

    • Biology for differential gene expression analysis - introduction
    • What is this thing called bioinformatics?
    • What is this thing called bioinformatics - quiz
    • Overview of Gene Expression
    • Translation of genetic code - theory
    • Translation of genetic code - practice
    • Open Reading Frame (ORF) translation
    • Open Reading Frame translation walk-through
    • Is genetic code redundant?
    • Gene structure and splicing
    • mRNA modifications
    • Biology for differential gene expression analysis - section summary

    Sequencing, PCR and Microarrays - Technical Foundation

    • Lab techniques for differential gene expression analysis - intro
    • Sequencing
    • PCR
    • Sanger sequencing
    • Next generation sequencing
    • Lab techniques for differential gene expression
    • Microarrays
    • Lab techniques for differential gene expression analysis - section summary

    Experimental, Statistical and analytical foundation plus quantitative PCR

    • First things first - RStudio installation
    • Differential Gene Expression - fundamentals
    • Sample preparation for differential gene expression analysis
    • Measuring gene expression with qPCR
    • qPCR quantification of gene expression
    • qPCR quiz - part 1 - intro
    • Is the expression of this tumour suppressor affected by this drug? - Part 1
    • qPCR quiz walkthrough - part 1
    • Experimental considerations for differential gene expression
    • Statistical considerations for differential gene expression
    • Spreadsheet qPCR analysis: preserving variability of expression
    • Data analysis in RStudio
    • Getting ready for your next quiz: dat package deprecation and functions
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q1
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q1
    • Part 2 - Q1 Walkthrough
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q2
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q2
    • Part 2 - Q2 walkthrough
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q3
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q3
    • Part 2 - Q3 walkthrough
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q4
    • Is the expression of this tumour suppressor affected by this drug? - Part 2 - Q3
    • Part 2 - Q4 walkthrough

    A Deep dive into RNAseq and analysis methods

    • Intro to RNAseq
    • Analytical advantages of RNAseq and overview of RNAseq analysis pipeline
    • RNAseq quick quizzes
    • RNAseq analysis pipeline
    • FastQC: assessing the quality of sequencing library
    • Your FastQC exercise
    • Should I trim this library of reads?
    • FastQC exercise walkthrough
    • Alignment (TUXEDO)
    • alignment (Rsubread)
    • Rsubread alignment quiz
    • Rsubread alignment: stringent vs. less stringent alignment criteria
    • Strict and less strict alignment quiz
    • Strict and less strict alignment walkthrough
    • Strict and less strict alignment quiz walkthrough
    • Transcriptome assembly (TUXEDO)
    • Installing Cuff-apps from TUXEDO toolkit
    • Comparing genome annotations with Cuffcompare
    • Cuffcompare genome annotation quiz
    • Cuffcompare quiz walkthrough
    • Read counts and FPKM/RPKM
    • FPKM quiz
    • Read counting (TUXEDO)
    • Read counts (Rsubread)
    • RPKM normalization (R)
    • Convert read counts to RPKMs
    • Convert read counts to RPKMs - quiz
    • Quiz 18 - Code to get the answers walkthrough
    • Differential gene expression (TUXEDO)
    • General Linear Models (GLMs) for differential gene expression analysis - part 1
    • GLMs for differential gene expression analysis - part 2
    • GLMs for differential gene expression analysis - part 3
    • GLMs for differential gene expression analysis - part 4
    • GLMs quiz
    • Design matrices for GLMs
    • Further read count processing for differential expression analysis
    • Differential expression (R's GLM): limma, edgeR
    • Differential expression: impact of filtering & normalization
    • Differential expression quiz
    • Differential expression quiz walkthrough
    • Differential expression: RankProd
    • Differential expression with RankProd in R
    • RankProd Quiz
    • RankProd Quiz walkthrough

    Pathway analysis for hypothesis generation

    • Hands on ORA-based pathway analysis
    • ORA Quiz
    • Hands on GSEA-based pathway analysis
    • GSEA Quiz
    • Using pathway analysis in hypothesis-free studies

    Gene Expression Analysis

    • Visualization of gene expression data
    • Using Venny for venn diagram analysis
    • Using Venny for venn diagram analysis - quiz
    • Learn to visualize gene expression data in R
    • Visualization of gene expression data hands on exercise

    Your Capstone project

    • Capstone project
    • Capstone project materials

    Instructors

    Mr Alexander Abdulkader Kheirallah

    Mr Alexander Abdulkader Kheirallah
    instructor
    Freelancer

    Other Bachelors, Ph.D

    Trending Courses

    Digital Marketing Courses Fashion Design Courses Data Science Courses Interior Design Courses Graphic Designing Courses Cyber Security Courses Nursing Courses Tally Courses Data Analysis Courses Web Designing Courses Artificial Intelligence Courses Ethical Hacking Courses Free Digital Marketing Courses And certifications Free Artificial Intelligence Courses And Certifications Free Data Analysis Courses And Certifications Free Cyber Security Courses And Certifications Free Data Science Courses And Certifications Free Cloud Computing Courses And Certifications Free Python Courses And Certifications Free Fashion And Textiles Courses And Certifications Free Graphic Designing Courses And Certifications Free Web Designing Courses And Certifications

    Popular Courses

    General Management Courses Public Health Courses Teaching and Education Courses Financial Management Courses Web Development Courses Mathematics Courses Data Science Courses Cyber Security Courses Programming Courses Digital Marketing Courses Law Courses Leadership Courses Explore all courses

    Popular Platforms

    upGrad Courses Udemy Courses Edx Courses Swayam Courses Coursera Courses Futurelearn Courses Mindmajix Technologies Courses Vskills Courses IIT Kharagpur Courses Emeritus Courses IIT Kanpur Courses NPTEL Courses Explore all platforms

    Learn more about the Courses

    10 Reasons to Enrol Yourself in a Digital Marketing Course 8 Must-Have Skills for AWS Cloud Architects Planning to Upskill Yourself? Enrol for a Program in Data Science 25+ Tips for Improving Your Graphic Design Skills Top Universities in India Offering Cyber Security Courses 15+ Courses for Learning Data Mining How to Make a Career in the Field of Artificial Intelligence Top 10 Benefits Of Holding A Certification In Business Intelligence Which are the best certification courses for Photography in India A Beginner's Guide to Pursue Python Programming Want to Pursue a Career in Blockchain Technology? Here is all that you need to Know How Entrepreneurs Can Use Machine Learning to Make their Business Successful? The Scope of Artificial Intelligence in India Top 10 Online Courses for Travel Lovers 10 Best Certification Courses After Hospital and Healthcare Management
    Providers
    Close

    Download the Careers360 App on your Android phone

    Regular exam updates, QnA, Predictors, College Applications & E-books now on your Mobile

    Careers360 App
    150M+ Students
    30,000+ Colleges
    500+ Exams
    1500+ E-books