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Quick Facts
| Medium Of Instructions | Mode Of Learning | Mode Of Delivery |
|---|---|---|
| English | Self Study | Text Based |
Courses and Certificate Fees
| Certificate Availability | Certificate Providing Authority |
|---|---|
| yes | RGITBT Pune |
The Syllabus
- Introduction. History. Scope. Advantages. Applications. Limitations.
- Guidelines for establishing academic and commercial laboratory.
- Steps involved in Plant Tissue Culture.
- Various nutrient medium composition.
- Plant growth regulators and their role in nutrient media.
- Types of organ cultures and their applications.
- Pathways of regeneration.
- Micrografting of commercially important plants. In vitro approaches for crop improvement.
- Plant cell reactors.
- Microtechniques
- Meristem culture for the production of virus free plants.
- Nucellus culture for clonal propagation and large scale multiplication.
- Stratagies of Micropropagation.
- Stages of micropropagation via axillary shoot proliferation in Monocots and Dicots.
- Stages in micropropagation via direct and indirect organogenesis.
- Stages in micropropagation via direct and indirect somatic embryogenesis.
- Low cost methods for micropropagation.
- Handling and Instrumentation of Plant Tissue Culture.
- Glassware Washing & Sterilization Techniques.
- Preparation of stock solutions and nutrient media.
- Surface sterilization of Explants.
- Monocot and Dicot Seed cultures for the establishment of organ cultures.
- Establishment of organ cultures for the induction of callus
- Establishment of organ cultures for the induction of multiple shoots
- Sub-culturing of nucellar seedlings for clonal propagation.
- Technique of micrograftingin fruit crops.
- Primary hardeningof tissue culture plants for their acclimatization
- Cytological study of calli cells and their subculturing
- Establishment of embryogenic/ non embryogenic cell suspension cultures.
- Embryo / Endosperm/ Ovules and anther Cultures.
- Demonstration of meristematic explants in different plants
- Preparation of mother plants for collection of explants
- Monocot shoot bud culture for axillary shoot proliferation
- Dicot Shoot tip / nodal segment culture for axillary shoot proliferation.
- Establishment of Dicot and Monocot multicultures
- In vitro and ex vitro rooting ofmicroshoots.
- Primary and secondary hardening of tissue cultured plants.
- Artificial seed production from microshootsand theirin vitro germination.
- Establishment of cultures for the induction of embryogenic callus.
- Maturation and conversion of somatic embryos into plantlets.
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