How recombinant DNA is formed ?
The process of recombinant DNA is:
It is formed by combining Ena fragments from different sources, typically using restricted enzymes to cut DNA at specific sequences, and after that joining those fragments by using DNA ligase.
Hello,
Reconbinant DNA is formed by combining DNA from two different sources using genetic engineering techniques. first, a gene of interest is isolated and inserted into a vector, like a plasmid using enzymes like restriction endonucleases. the recombinant vector is then introduced into a host cell, where the foreign DNA can be replicated or expressed.
Hello,
Recombinant DNA (rDNA) is formed by combining genetic material from two different sources. The process involves:
1. Isolation of DNA – The desired gene is extracted from an organism.
2. Cutting DNA – Both the target DNA and vector DNA (like plasmid) are cut using restriction enzymes.
3. Ligation- The gene is inserted into the vector using DNA ligase.
4. Transformation – The recombinant DNA is introduced into a host cell for replication.
Hope this helps you,
Thank you
Recombinant DNA is formed by combining DNA from two different sources. The process involves:
-
Isolation of DNA – The desired gene is extracted from an organism’s DNA.
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Cutting DNA – Both the gene and a vector (like a plasmid) are cut using restriction enzymes.
-
Ligation – The gene is inserted into the vector using DNA ligase.
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Transformation – The recombinant DNA is introduced into a host cell (e.g., bacteria).
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Expression – The host cell produces the desired protein.
This technique is widely used in medicine, agriculture, and research.