how temperature that pollengrain become active in lequede nitrogen
Answer (1)
25th Nov, 2018
Dear,
Just be sure that you freeze mature, fully dehydrated Lilium pollen grains directly in tubes filled with liquid N2. We are punching a small hole into the tube lid, so the vials don't explode in the -80C freezer if there are rests of liquid N2 in the tube. Using this method, lily pollen showed germination and tube growth even after more than 10 years!
Thanks
Just be sure that you freeze mature, fully dehydrated Lilium pollen grains directly in tubes filled with liquid N2. We are punching a small hole into the tube lid, so the vials don't explode in the -80C freezer if there are rests of liquid N2 in the tube. Using this method, lily pollen showed germination and tube growth even after more than 10 years!
Thanks
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