Hello,
Your graduation degree will still be valid even if you repeat class 12th from National Institute of Open Schooling (NIOS). But you cannot utilize NIOS certificate and previous board class 12th certificate together. Only one board certificate is considered at time of admission in to the course you are seeking admission, it will be either NIOS certificate or previous board certificate depending on the certificate you are providing as you are appearing for all the all 5 subjects through NIOS. So you can provide only one class 12th certificate that is NIOS to get admission in to a desired course.
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