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Principles Of Biotechnology

Isolation of the DNA from the donor organism. DNA fragmentation using the restriction endonucleases. Ligation of the desired DNA fragment into the vector. Recombinant DNA is then transferred to the host.

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There are two principles of biotechnology

• The principle of genetic engineering is to modify the existing organisms by changing the genetic material in them. It mainly includes the recombinant DNA technology. The technique of altering the chemistry of DNA and RNA
• Maintenance of sterile conditions to enable growth of only the desired microbe or eukaryotic cell in large quantities for the manufacture antibiotics, vaccines, enzymes, etc.