Which DNA - GC rich or AT rich DNA has higher melting point and why?
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Melting point of double stranded DNA means the temperature at which the two strands separate by breakage of the Hydrogen bonds between the purine and pyrimidine bases. As G and C residues are joined by three Hydrogen bonds whereas A and T residues are joined by two Hydrogen bonds, the energy required to separate the strands of any DNA rich in G-C bases is more than that required to separate the strands of any A-T rich sequence. So, G-C content of the ds DNA is the most important factor for determining the melting temperature of DNA.
I hope this helps !
Hi Ram Mohan Nayak !
GC rich DNA has higher melting point. Because G-C pairs form three hydrogen bonds, while A-T pairs form only two, the higher the percentage of GC content, the higher its melting temperature. Thus, A double-stranded DNA rich in G and C needs more energy to be broken than one that is rich in A and T .
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